Laboratory Protocols
 

HISTOLOGY

Paraffin Embedding

Massons Trichrome

Saffranin O/Fast Green

PROTEIN QUANTIFICATION

NP-40 Lysis Buffer

RIPA Buffer

HALT Protease Inhibitor

LICOR Westerm Blot

GENE QUANTIFICATION

TRIzol

QIAzol Lysis Reagent

RNEasy Part 1

RNEasy Part 2

QIAshredder

Clontech RNA to cDNA EcoDry Premix

qRT-PCR

Primer Design

IMMUNOFLUORESCENCE

Confocal Immunofluorescence

Proximity Ligation Assay

Whole Mount Immuno

OTHER

Transmission Electron Microscopy

General Reagents

David Reed, Reed Lab, The University of Illinois at Chicago (2015)